Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Primary alloproliferative TH1 response induced by immature plasmacytoid dendritic cells in collaboration with myeloid DCs.

doi: 10.1111/j.1600-6143.2005.01097.x

Figure Lengend Snippet: Figure 4: The phenotype of the responder T-cell population is similar in MDC and 5000PDC+100MDC allostimulatory conditions. After 5 days of culture, the alloproliferating CFSE-low T cells were stained with different mAbs to the indicated markers. (A) The expression of IL2Ra, IL12Rb1 and CD154 on proliferating T cells (gated on R1) is shown. The numbers indicate the percentage of cells in each quadrant. (B) Summary of the markers analyzed and detected (+ve) on responding CFSE-low T cells stimulated by 5000MDC (black bars) or 5000PDC+100MDC (white bars) (mean ± SD, n = 4). As most cells expressing the markers are CFSE low, the proportion is calculated based on the proliferating cells (contained in the R2 regions). (C) The time-dependent expression of IL12Rb2 on CFSE-low T cells was analyzed in three different culture experiments stimulated by (5000PDC+100MDC) (white symbols) or 5000MDCs alone (black symbols) for the indicated time period.

Article Snippet: The following murine monoclonal antibodies (mAbs) were used: Peridinin Chlorophyll Protein (PerCP)-labeled CD3 and HLA-DR; Fluorescein isothiocyanate (FITC)-labeled CD4 and CD45RA; Phycoerythrin (PE)-labeled CD11c, CD123, IL2Ra, CD154, CD45RO, IL12Rb1, IL12Rb2, IL18Ra (BD Biosciences Oxford, UK); TRI-Color® (TC)-labeled CD3, PE-labeled IL4, Allophycocyanin (APC)-labeled IFNc (Caltag Laboratories, Hamburg, Germany); FITC-labeled anti-BDCA2 (Miltenyi Biotec, Bergisch Gladbach, Germany); RPE-Cy5-labeled CD14, CD19 (Serotec Ltd, Kidlington, Oxford, UK).

Techniques: Staining, Expressing

Journal: Biomolecules

Article Title: Short Carbon Nanotube-Based Delivery of mRNA for HIV-1 Vaccines

doi: 10.3390/biom13071088

Figure Lengend Snippet: The percentages of human CD45+ cell repopulation, as well as the reconstitution status of various human immune competent cells, in the peripheral blood of AAV9-transduced NSG-B2m KO mice.

Article Snippet: A few hours later, the NSG-B2m KO mice were engrafted intravenously with 1 × 10 5 human hematopoietic stem cells (HSCs), which were purified by CD34+ beads (Miltenyi, San Diego, CA, USA) from human cord blood cells.

Techniques:

Journal: Cells

Article Title: Synergistic Effects of N-Acetylcysteine and Mesenchymal Stem Cell in a Lipopolysaccharide-Induced Interstitial Cystitis Rat Model

doi: 10.3390/cells9010086

Figure Lengend Snippet: Human antigens in transplanted M-MSCs. ( a ) Representative immunofluorescence micrographs of bladder sections of LPS-IC + 25K M-MSC rats stained for hB2M (green) structures (magnification ×1000, scale bar = 10 μm). ( b ) Representative immunofluorescence micrographs of bladder sections of LPS-IC + 50K M-MSC rats stained for hB2M (green) structures (magnification ×1000, scale bar = 10 μm). Nuclei were stained with DAPI (blue). ( c ) Number of transplanted M-MSCs (hB2M positive cells) in urothelium and serosa. DAPI: 4’,6-diamidino-2-phenylindole

Article Snippet: Engraftment of the administered M-MSCs was determined by immunofluorescence analysis (EVOS, FL Color Imaging System, Life Technologies, Carlsbad, CA, USA) of human β2-microglobulin (hB2M) (mouse monoclonal, SC80668; Santa Cruz Biotechnology Inc., Paso Robles, CA, USA) and visualized by Alexa 488-conjugated (A11029) anti-mouse antibody (Molecular Probes, Grand Island, NY, USA), as previously described [ , ].

Techniques: Immunofluorescence, Staining

Journal: Molecular Neurodegeneration

Article Title: Enhancing GABAergic signaling ameliorates aberrant gamma oscillations of olfactory bulb in AD mouse models

doi: 10.1186/s13024-021-00434-7

Figure Lengend Snippet: Information of the primary antibodies used in this study

Article Snippet: GABAAR-β2 , Abcam , ab156000 , Rabbit , , 1:10000.

Techniques: